Application of Whole Genome Sequencing to Antibiotic Resistance Surveillance and Outbreak Investigations


Kelsey Florek, MPH, PhD
2019 APHL Annual Meeting
June 04, 2019


Slides available at:
www.k-florek.net/talks

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APHL - CDC Antimicrobial Resistance Fellowship

Bioinformatics Pipeline

got to work

collaborations

  • Wisconsin Department of Public Health
  • Wisconsin Veterinary Diagnostic Laboratory
  • ORegon CHild Absenteeism due to Respiratory Disease Study (ORCHARDS)
  • Strategic planning for sequencing and bioinformatics at the State Laboratory

Carbapenem Resistant Acinetobacter baumannii outbreak

Antimicrobial susceptibility

  • Amikacin: Resistant
  • Aztreonam: Resistant
  • Cefepime: Resistant
  • Cefotaxime: Resistant
  • Ceftazidime: Resistant
  • Ciprofloxacin: Resistant
  • Colistin: Susceptible
  • Doripenem: Resistant
  • Doxycycline: Resistant
  • Gentamicin: Resistant
  • Imipenem: Resistant
  • Levofloxacin: Resistant
  • Meropenem: Resistant
  • Minocycline: Intermediate
  • Piperacillin-tazobactam: Resistant
  • Polymyxin-B: Susceptible
  • Ticarcillin-clavulanate: Resistant
  • Tobramycin: Resistant
  • Trimethoprim-sulfamethoxazol: Resistant

P-094

Carbapenem-resistant Acinetobacter baumannii Harboring a Plasmid Carrying blaOXA-72 in an Outbreak Involving an Intensive Care Unit and Long-term Care Facilities in Wisconsin

Conclusions

  • Chance favors the prepared mind. - Louis Pasteur
  • Expand laboratory capacity
  • Work in an area I love

Acknowledgements

  • Wisconsin State Laboratory of Hygiene
    • David Warshauer
    • Peter Shult
    • Allen Bateman
    • Alana Sterkel
    • Sara Wagner
  • Wisconsin Department of Public Health
    • Megan Lasure
    • Lina Elbadawi
    • Jordan Mason
    • Rachel Klos
  • Association of Public Health Laboratories
    • Nikki Marchan
    • Kelly Wroblewski
    • Christin Hanigan

special thanks to: